Allevi Publications

Allevi 2 Publications  3D printed UV light cured polydimethylsiloxane devices for drug delivery This paper, published by researchers from  Åbo Akademi University and the University of Helsinki in Finland, use the Allevi 2 platform to fabricate drug-containing PDMS structures. Abstract: The goal of this work was to study the printability of PDMS with a semi-solid extrusion Read More

Troubleshooting Issues With 3D Culture Viability

Nothing is quite as frustrating as an unexpected loss of viability when working with 3D cultures. Below is a troubleshooting guide that outlines the most common variables affecting viability in 3D cultures and what steps to take to create viable, healthy 3D bioprinted cultures. Part 1: General 3D Culture Variables Before focusing on parameters in the Read More

Modeling Thrombosis with Sacrificial Bioprinting

Thrombosis constitutes a major reason for morbidity and mortality in cardiovascular diseases and its complications. An in vitro thrombosis model has been recently built by taking advantage of an optimized sacrificial bioprinting strategy [1]. Using a bioprinter (BioBot Beta), i, ii) a template and a wall is printed with 40% Pluronic F127 aqueous solution, followed Read More

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Educating Students to Build with Biology

Building with Biology For generations, the “makers” of the world have been building increasingly complex machines and systems with traditional materials such as woods, metals, ceramics, and plastics. These materials have many advantageous properties, but they all have one disadvantage: they do not dynamically sense and adaptively respond to their environments in real-time. However, we Read More

Stanford Researchers Develop Novel Bioink

Figure (adapted):  A) Schematic depicting the drawbacks of commonly used bioinks for each stage of the printing process. These drawbacks can be overcome by new biomaterials development. B) Schematic of the two material components of MITCH-Alginate and its dual-stage crosslinking. In the first stage, noncovalent binding between two complementary peptides forms a weak gel upon mixing Read More

Bioinks for Biofabrication: Current State and Future Perspectives

Figure from Wake Forest University. Recent progress in 3D printing technologies is leading a revolution in cell culture methods. These systems design complex physiologically relevant 3D environments. 3D culture technologies rely heavily on bioinks, the raw cells and biomaterials used to create these 3D cultures. These bioinks, ranging from cell suspensions to hard acellular thermoplastics, Read More

Integrated Tissue-Organ Printer

An integrated tissue-organ printer, referred to as an ITOP, takes bioprinting to the next level by allowing for the fabrication of scalable tissues, such as cartilage, bone, and skeletal muscle (1). Figure 1: (from Wake Forest University) Left: The ITOP printing into a petri dish.  Right: A 3D printed bone segment and ear lay in Read More

Effects of Printing Shear Stress on Viability

From stem cell differentiation to cell signaling and protein expression, shear stress acts as an important biomechanical stimuli in cell biology (1-3). However, too much shear stress can lead to apoptosis or necrosis (1-3). Shear stress during bioprinting plays a large role in final viability of a construct and must be minimized. Therefore, it is necessary to identify the Read More

FRESH Bioprinting Method

Figure 1: Kidney (left) and bifurcated tube (right) printed using a BioBot 1 and the FRESH method. Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). The method utilizes a support hydrogel as a temporary, thermoreversible support Read More

Matrix Bioinks

Just as a 2D printer has a variety of color inks to create an assortment of images, bioprinters utilize a variety of bioinks to create an assortment of viable 3D environments.  One of the first steps in the bioprinting process involves choosing what inks to print. Figure 1: Many different types of bioinks are used to Read More