Common Viability Assays for 3D Cultures

As the 3D culture revolution takes over biology, new methods must be developed to analyze these cultures. Quantitative viability assays for 3D cultures are lacking, and even the ones that are available have only been tested by manufacturers with spheroid cultures, as opposed to larger cell-encapsulated hydrogel cultures. However, there are some current methods for viability analysis that Read More

Processed Gelatin/Fibrin Bioprinted Viability

Introduction Matrix bioinks, or cell-encapsulating bioinks, are arguably the most important reagents used in the bioprinting processes. These materials must shield cells from shear stresses during the printing process, closely mimic the extracellular matrix and offer quick, nontoxic gelation for optimal print resolution. Types of matrix bioinks range from simple cell slurries suspended in media Read More

Percoll Improves Bioprinted Cell Distribution

Percoll is a well-referenced media for density gradient centrifugation of cells, viruses and subcellular particles(1). Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll has also been previously used to improve cell distribution when bioprinting with low viscosity matrix bioinks(2). Below, Percoll is tested with two BioBots reagents, PEGDA and fibrinogen. Results PEGDA Read More

Bioprinted GelMA Viability

Introduction Gelatin methacrylate, a common photopolymerizable biomaterial, is a gelatin-based hydrogel modified with a methacrylate group that crosslinks through free radical polymerization. Derived from collagen, gelatin methacrylate exhibits many native properties of the extracellular matrix (ECM), including the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs (1,2). As a photopolymerizable hydrogel with tunable mechanical Read More

Bioprinted Alginate Viability

Introduction As a naturally occurring polysaccharide commonly derived from algae or seaweed, sodium alginate’s abundance and low cost make it a popular biomaterial for cellular encapsulation and bioprinting (1). Sodium alginate forms a hydrogel through a sodium-calcium ion exchange. As this crosslinking process occurs very quickly, sodium alginate can be difficult to bioprint on its own. Many studies combine Read More

LAP and Irgacure Viability with GelMA

Introduction Photopolymerization is a common process used for the solidification of materials in a variety of applications including dentistry, electronics, printing, optics and tissue engineering (2). This process, which allows for the fabrication of cell-laden hydrogels from aqueous precursors, offers advantages such as spatial and temporal control, fast curing rates at physiological conditions and minimal Read More

PCL Viability

Thermoplastics in bioprinting are often used as a support structure to provide extra mechanical strength and as reinforcement for matrix bioinks[1,2]. Polycaprolactone is a biodegradable thermoplastic used in a variety of medical applications, including bioprinting. This study tested the viability and print resolution of  polycaprolactone, or PCL, with the BioBot 1. PCL Print Resolution Figure Read More