Collagen and FRESH printing

Overview Figure 1:  Live/dead image of human neonatal dermal fibroblasts encapsulated in LifeInk200 (collagen) after 1 day of culture. LifeInk200 is a high concentration collagen bioink that can be thermally crosslinked at temperatures above 10ºC. Due to its concentration, it has impressive shape fidelity during bioprinting. However, since LifeInk200 cannot support itself before crosslinking, we Read More

Modeling Thrombosis with Sacrificial Bioprinting

Thrombosis constitutes a major reason for morbidity and mortality in cardiovascular diseases and its complications. An in vitro thrombosis model has been recently built by taking advantage of an optimized sacrificial bioprinting strategy [1]. Using a bioprinter (BioBot Beta), i, ii) a template and a wall is printed with 40% Pluronic F127 aqueous solution, followed Read More

BioBots Reagent Guide

Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in Read More


Case Study: Hydroxyapatite and PCL

Hydroxyapatite and polycaprolactone blends have been previously investigated as scaffolds for bone tissue engineering [1]. In addition to its biocompatibility, hydroxyapatite offers osteoconductive properties. However, with a high melting temperature and insolubility in water, hydroxyapatite presents challenges for extrusion bioprinting. When mixed with polycaprolactone, which exhibits a low melting point, the blended materials can be Read More

Gelatin Printing

Gelatin is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. Through a reversible thermal gelation, gelatin remains solid at room temperature and can be melted away at 37°C.  The recommended use for gelatin as a sacrificial material is as a FRESH support material. Below are results Read More

Sodium Alginate and Calcium Chloride

Note: The best method for printing with cell-encapsulated sodium alginate is the FRESH method. This simplified method below will allow you to fabricate thin films and very simple constructs with limited resolution. We highly suggest the FRESH method for any complex bioprinting or any bioprinting that requires high resolution with sodium alginate. Overview Figure 1:  Live/dead Read More

FRESH Bioprinting Method

Figure 1: Kidney (left) and bifurcated tube (right) printed using a BioBot 1 and the FRESH method. Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). The method utilizes a support hydrogel as a temporary, thermoreversible support Read More

Pluronic F127

Overview Figure 1(1):  An engineered tissue construct of bioprinted cell-laden GelMA and sacrificial Pluronic F127. Pluronic F127 is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. The recommended preparation provided in the user instruction below yields a streamlined printable biomaterial that supports 3D printed cellular constructs. However, Read More