BioBots Reagent Guide

Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in Read More

Percoll Improves Bioprinted Cell Distribution

Percoll is a well-referenced media for density gradient centrifugation of cells, viruses and subcellular particles(1). Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll has also been previously used to improve cell distribution when bioprinting with low viscosity matrix bioinks(2). Below, Percoll is tested with two BioBots reagents, PEGDA and fibrinogen. Results PEGDA Read More

PEGDA

Overview Figure 1:  Live/dead image of human neonatal dermal fibroblasts encapsulated in bioprinted PEGDA Poly(ethyelene glycol) diacryate is a PEG-based hydrogel that reacts and crosslinks in the presence of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and BioBots blue light technology(1,2). The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that supports 3D printed cell-laden constructs. However, Read More

Bioprinted PEGDA Viability

Introduction Poly(ethyelene glycol) diacryate is one of the most common synthetic hydrogels used for cell encapsulation. Advantages of this biomaterial include biocompatibility, hydrophilicity and the ability to chemically tailor the material (1-3). This study tests the viability of bioprinted PEGDA (MW 3400), Percoll and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) with a BioBots printer. Results Crosslinking Viability  First, a variety Read More