Common Viability Assays for 3D Cultures

As the 3D culture revolution takes over biology, new methods must be developed to analyze these cultures. Quantitative viability assays for 3D cultures are lacking, and even the ones that are available have only been tested by manufacturers with spheroid cultures, as opposed to larger cell-encapsulated hydrogel cultures. However, there are some current methods for viability analysis that Read More

Creating 3D Controls: Bioprint Study

New to the world of 3D biology, or testing out a new matrix material with your BioBot? This protocol is part of a series to help you learn how to design experiments and easily build with life! Before you begin bioprinting, you need to make sure you have proper controls. Typical 2D culture controls are Read More

Troubleshooting Issues With 3D Culture Viability

Nothing is quite as frustrating as an unexpected loss of viability when working with 3D cultures. Below is a troubleshooting guide that outlines the most common variables affecting viability in 3D cultures and what steps to take to create viable, healthy 3D bioprinted cultures. Part 1: General 3D Culture Variables Before focusing on parameters in the Read More

Soft Tissue Kit Protocol

Overview Figure 1:  Live/dead image of human neonatal dermal fibroblasts encapsulated in bioprinted Gelatin/Fibrin Processed Gelatin/Fibrin Soft Tissue Kit contains all the materials needed to print with this gelatin-based hydrogel, which solidifies through enzymatic crosslinking with a thrombin, calcium chloride, and transglutaminase solution. The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that Read More

Processed Gelatin/Fibrin Bioprinted Viability

Introduction Matrix bioinks, or cell-encapsulating bioinks, are arguably the most important reagents used in the bioprinting processes. These materials must shield cells from shear stresses during the printing process, closely mimic the extracellular matrix and offer quick, nontoxic gelation for optimal print resolution. Types of matrix bioinks range from simple cell slurries suspended in media Read More

Bioprinted Collagen Viability

Introduction LifeInk200 is a high concentration collagen bioink useful for bioprinting structures with high shape fidelity. As the most common protein in many tissues’ ECMs, collagen-based bioinks are great for cell encapsulation because they demonstrate increased cell adhesion (1). As a result of thermal crosslinking, the collagen molecules form fibrils and then more stable and Read More

Printing with GelMA: Troubleshooting

Below are some common issues users have when printing with GelMA and potential troubleshooting steps. For more details on this material, check out some background information, our protocol or our bioreport. Issue 1: My GelMA solution seems to have a low viscosity, or quickly dispenses out of my syringe. Troubleshooting tip 1: Try placing your syringe on ice Read More

Modeling Thrombosis with Sacrificial Bioprinting

Thrombosis constitutes a major reason for morbidity and mortality in cardiovascular diseases and its complications. An in vitro thrombosis model has been recently built by taking advantage of an optimized sacrificial bioprinting strategy [1]. Using a bioprinter (BioBot Beta), i, ii) a template and a wall is printed with 40% Pluronic F127 aqueous solution, followed Read More

Creating 3D Controls: Encapsulation Study

New to the world of 3D biology, or testing out a new matrix material with your BioBot? This protocol is the first of a series to help you learn how to design experiments and easily build with life! Before you begin bioprinting, you need to make sure you have proper controls. Typical 2D culture controls Read More

BioBots Reagent Guide

Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in Read More