Processed Gelatin/Fibrin Bioprinted Viability

Introduction Matrix bioinks, or cell-encapsulating bioinks, are arguably the most important reagents used in the bioprinting processes. These materials must shield cells from shear stresses during the printing process, closely mimic the extracellular matrix and offer quick, nontoxic gelation for optimal print resolution. Types of matrix bioinks range from simple cell slurries suspended in media Read More

Bioprinted Collagen Viability

Introduction LifeInk200 is a high concentration collagen bioink useful for bioprinting structures with high shape fidelity. As the most common protein in many tissues’ ECMs, collagen-based bioinks are great for cell encapsulation because they demonstrate increased cell adhesion (1). As a result of thermal crosslinking, the collagen molecules form fibrils and then more stable and Read More

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Case Study: Hydroxyapatite and PCL

Hydroxyapatite and polycaprolactone blends have been previously investigated as scaffolds for bone tissue engineering [1]. In addition to its biocompatibility, hydroxyapatite offers osteoconductive properties. However, with a high melting temperature and insolubility in water, hydroxyapatite presents challenges for extrusion bioprinting. When mixed with polycaprolactone, which exhibits a low melting point, the blended materials can be Read More

Percoll Improves Bioprinted Cell Distribution

Percoll is a well-referenced media for density gradient centrifugation of cells, viruses and subcellular particles(1). Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll has also been previously used to improve cell distribution when bioprinting with low viscosity matrix bioinks(2). Below, Percoll is tested with two BioBots reagents, PEGDA and fibrinogen. Results PEGDA Read More

Bioprinted PEGDA Viability

Introduction Poly(ethyelene glycol) diacryate is one of the most common synthetic hydrogels used for cell encapsulation. Advantages of this biomaterial include biocompatibility, hydrophilicity and the ability to chemically tailor the material (1-3). This study tests the viability of bioprinted PEGDA (MW 3400), Percoll and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) with a BioBots printer. Results Crosslinking Viability  First, a variety Read More

Gelatin Printing

Gelatin is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. Through a reversible thermal gelation, gelatin remains solid at room temperature and can be melted away at 37°C.  The recommended use for gelatin as a sacrificial material is as a FRESH support material. Below are results Read More

PLGA Viability

Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradable and mechanical properties[1,2]. The degradation and mechanical properties of this material can be altered by adjusting the ratios of the copolymers polylactide and polyglycolide [1,2]. Thermoplastics in bioprinting are often used as a support structure to provide extra mechanical strength Read More

Bioprinted GelMA Viability

Introduction Gelatin methacrylate, a common photopolymerizable biomaterial, is a gelatin-based hydrogel modified with a methacrylate group that crosslinks through free radical polymerization. Derived from collagen, gelatin methacrylate exhibits many native properties of the extracellular matrix (ECM), including the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs (1,2). As a photopolymerizable hydrogel with tunable mechanical Read More

Bioprinted Alginate Viability

Introduction As a naturally occurring polysaccharide commonly derived from algae or seaweed, sodium alginate’s abundance and low cost make it a popular biomaterial for cellular encapsulation and bioprinting (1). Sodium alginate forms a hydrogel through a sodium-calcium ion exchange. As this crosslinking process occurs very quickly, sodium alginate can be difficult to bioprint on its own. Many studies combine Read More

World Biomaterials Congress 2016 Poster Presentations

BioBots presented two posters at the World Biomaterials Congress in Montreal. Check out the posters and introductions below. BioBots for Standardizing the 3D Bioprinting of Bioinks Introduction: Developments in 3D bioprintingtechnologies and biomaterials allow for the enhanced fabrication of 3D culture environments with complex geometries and designs.1,2 While a variety of 3D printers are commercially available Read More