BioBots Reagent Guide

Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in Read More

Stanford Researchers Develop Novel Bioink

Figure (adapted):  A) Schematic depicting the drawbacks of commonly used bioinks for each stage of the printing process. These drawbacks can be overcome by new biomaterials development. B) Schematic of the two material components of MITCH-Alginate and its dual-stage crosslinking. In the first stage, noncovalent binding between two complementary peptides forms a weak gel upon mixing Read More

Bioprinted Alginate Viability

Introduction As a naturally occurring polysaccharide commonly derived from algae or seaweed, sodium alginate’s abundance and low cost make it a popular biomaterial for cellular encapsulation and bioprinting (1). Sodium alginate forms a hydrogel through a sodium-calcium ion exchange. As this crosslinking process occurs very quickly, sodium alginate can be difficult to bioprint on its own. Many studies combine Read More

Sodium Alginate and Calcium Chloride

Note: The best method for printing with cell-encapsulated sodium alginate is the FRESH method. This simplified method below will allow you to fabricate thin films and very simple constructs with limited resolution. We highly suggest the FRESH method for any complex bioprinting or any bioprinting that requires high resolution with sodium alginate. Overview Figure 1:  Live/dead Read More

FRESH Bioprinting Method

Figure 1: Kidney (left) and bifurcated tube (right) printed using a BioBot 1 and the FRESH method. Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). The method utilizes a support hydrogel as a temporary, thermoreversible support Read More