Below are directions for a sample dual extrusion bioprint. This protocol is meant for educational purposes to help you learn how to use your BioBot 1 and familiarize yourself with the bioprinting process in general.
Load Your Syringe
- Remove Pluronic F127 from storage. The material should be a liquid solution. While still cold, load your syringe with the material. For this first print, we suggest loading 2-3 ml.
- First place a syringe cap on your syringe.
- Then gently pour or pipette Pluronic F127 into the syringe
- Add the stopper, then invert the syringe and remove the syringe cap.
- Carefully push the material to the top of the syring with a lab marker, then cap with a 30 gauge needle.
- Place your syringe into your BioBot. Allow Pluronic F127 to warm to room temperature before printing.
- Finally, place a petri dish into the bed plate.
Set Up Your Software
- Open Repetier Host and load your CAD File(Figures 1-5). We suggest this dual concentric file.
- For bioprints with Pluronic F127, you will want to set the slicer and print parameters to match the settings below.
|Speed (mm/s)||Layer height (mm)||Nozzle Diam (mm)||Gauge|
|Pressure (psi)||Crosslink (sec)||Print Temp (°C)|
3.Slice your design and save the gcode file.
4. Upload your file to the BioBots software.
- Connect your computer to your BioBot.
- Using software and manual controls, calibrate your BioBot to determine the zero positions.
Figure 1: Upload your .stl files into Repetier host. You should have one file for extruder 1 and one file for extruder 2. Rotate and orient properly.
Figure 2: Place you files on the bed and center them.
Figure 3: Label each file with the correct extruder for printing. Delta 1 refers to the left extruder on the BioBot 1. Delta 2 refers to the right extruder on the BioBot 1.
Figure 4: Slice the file with desired slicing parameters. If a warning pops up that at least one object is outside the printable area, press no.