Easily Mix Bioinks: The SpeedMixer

Have trouble preparing highly viscous bioinks? Need a tool to mix two components of a bioink with varying viscosities? Or do you need to mix a powder with a viscous solution? Does it take you forever to mix components used in your bioink? If so, we suggest checking out the FlackTek SpeedMixer. We’ve been testing the FlackTek SpeedMixer in our lab here Read More

Common Viability Assays for 3D Cultures

As the 3D culture revolution takes over biology, new methods must be developed to analyze these cultures. Quantitative viability assays for 3D cultures are lacking, and even the ones that are available have only been tested by manufacturers with spheroid cultures, as opposed to larger cell-encapsulated hydrogel cultures. However, there are some current methods for viability analysis that Read More

Troubleshooting Issues With 3D Culture Viability

Nothing is quite as frustrating as an unexpected loss of viability when working with 3D cultures. Below is a troubleshooting guide that outlines the most common variables affecting viability in 3D cultures and what steps to take to create viable, healthy 3D bioprinted cultures. Part 1: General 3D Culture Variables Before focusing on parameters in the Read More

Modeling Thrombosis with Sacrificial Bioprinting

Thrombosis constitutes a major reason for morbidity and mortality in cardiovascular diseases and its complications. An in vitro thrombosis model has been recently built by taking advantage of an optimized sacrificial bioprinting strategy [1]. Using a bioprinter (BioBot Beta), i, ii) a template and a wall is printed with 40% Pluronic F127 aqueous solution, followed Read More

BioBots Reagent Guide

Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1. Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in Read More


Educating Students to Build with Biology

Building with Biology For generations, the “makers” of the world have been building increasingly complex machines and systems with traditional materials such as woods, metals, ceramics, and plastics. These materials have many advantageous properties, but they all have one disadvantage: they do not dynamically sense and adaptively respond to their environments in real-time. However, we Read More

Stanford Researchers Develop Novel Bioink

Figure (adapted):  A) Schematic depicting the drawbacks of commonly used bioinks for each stage of the printing process. These drawbacks can be overcome by new biomaterials development. B) Schematic of the two material components of MITCH-Alginate and its dual-stage crosslinking. In the first stage, noncovalent binding between two complementary peptides forms a weak gel upon mixing Read More

Gelatin Methacrylate Review

Gelatin methacrylate, commonly referred to as gelMA, is a photocrosslinkable natural bioink derived from a hydrolytic degradation of collagen (1). GelMA is commonly used in drug delivery systems and wound dressing applications (1). With its cell encapsulation properties and thixotropic nature, gelMA is also a common reagent used for bioprinting (8). It has been used Read More

Reagent Development for Bioprinting

The BioBot is a versatile printer capable of printing with a wide array of materials and cells types. In addition to pre-optimized materials, many users also apply their own reagents with the BioBot. Below is an overview of reagent development used by the research team at BioBots. This general development process can be followed when Read More

Bioinks for Biofabrication: Current State and Future Perspectives

Figure from Wake Forest University. Recent progress in 3D printing technologies is leading a revolution in cell culture methods. These systems design complex physiologically relevant 3D environments. 3D culture technologies rely heavily on bioinks, the raw cells and biomaterials used to create these 3D cultures. These bioinks, ranging from cell suspensions to hard acellular thermoplastics, Read More