Revision for “PCL” created on August 22, 2016 @ 14:16:35

<h3><b>Overview</b></h3> Polycaprolactone (PCL) is specifically developed and optimized for bioprinting of 3D structures. PCL is a thermoplastic polymer that offers enhanced control over mechanical properties of final 3D structures. PCL may be used on its own or printed with matrix bioinks such as <a href="https://www.biobots.io/wiki/biogel/" target="_blank">gelatin methacrylate</a>. <img class="alignnone wp-image-482" src="https://www.biobots.io/wp-content/uploads/2016/02/pcl-400x266.png" alt="BioPCL lattice printed with a BioBot 1" width="336" height="223" /><img class="alignnone wp-image-483" src="https://www.biobots.io/wp-content/uploads/2016/02/pcl-alg-supp-and-fill-400x198.png" alt="BioPCL and alginate structure" width="452" height="223" /> <h6 style="text-align: center;">Figure 1: PCL can be printed on its own (left) or in combination with a matrix bioink (right) for additional support[1].</h6> The recommended preparation provided in the user instruction below yields a streamlined printable biomaterial that creates  reproducible 3D printed constructs. However, preparation can be modified by users to suit their needs. <h3><b>Storage </b><b>and </b><b>Handling</b></h3> PCL should be stored at room temperature. <h3>You Will Need</h3> <ul> <li style="margin-left: 20px;"> Metal needles (we suggest <a href="http://www.ellsworth.com/products/dispensing-equipment-supplies/tips-needles-nozzles-static-mixers/nozzles/fisnar-5901008-micron-s-precision-standard-bore-nozzle-black-30-ga/" target="_blank">these</a>)</li> <li style="margin-left: 20px;"><a href="https://www.biobots.io/accessories/" target="_blank">Stainless Steel 10 ml BioBots Syringe</a></li> <li style="margin-left: 20px;">Petri dish or well plate for printing</li> </ul> <h3><b>Instructions</b> <b>for </b><b>Use</b></h3> <h6>*If printing with cell-laden matrix bioinks, all steps must be completed in a sterile environment. Be sure to sterilize PCL overnight prior to use with cells.</h6> <ol> <li style="margin-left: 20px;">If using with cells, first sterilize PCL. <ol> <li style="margin-left: 20px;">Soak PCL in 70% ethanol overnight</li> <li style="margin-left: 20px;">After overnight ethanol wash, complete four 30 minute washes in sterile PBS to remove any leftover traces of ethanol</li> <li style="margin-left: 20px;">Allow PCL to dry before loading into syringge</li> </ol> </li> <li style="margin-left: 20px;"> Measure the desired amount of PCL for printing and load into metal syringe.</li> <li style="margin-left: 20px;"> Cap the metal syringe with a metal needle. A 30 gauge needle is suggested.</li> <li style="margin-left: 20px;">Load syringe into the first extruder in the BioBots printer. Connect to your BioBots printer through your computer.</li> <li style="margin-left: 20px;">Set the heat for the first extruder to suggested print temperature and allow time for PCL to melt.</li> </ol> <h3><b>Print Settings</b></h3> <p style="text-align: left;">[table caption="Slic3r Settings" style="text-align:center"] Speed (mm/s), Layer height (mm), Nozzle Diam (mm),   Gauge</p> <p style="text-align: center;">1.0, 0.1, 0.1, 30[/table]</p> [table caption= "Print Settings" style="text-align:center"] Pressure (psi), Crosslink (sec),Print Temp (°C) <p style="text-align: center;">100,0,100[/table]</p> <h3>Try it out!</h3> Try a practice print by following the instructions for <a href="https://www.biobots.io/wiki/your-first-bioprint/">Your First Bioprint</a> using the print parameters and loading instructions for PCL. <h3><b>Notes</b></h3> <ol> <li style="margin-left: 20px;">Print speed, nozzle diameter and pressure may all be adjusted to better fit a user’s needs. For help adjusting print parameters please contact <u>info@biobots.io.</u></li> <li style="margin-left: 20px;">A fill volume change of more than 2 ml may affect pressure settings.</li> <li style="margin-left: 20px;">A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution</li> </ol> <h3>Further Information</h3> <ul> <li style="margin-left: 20px;"><a href="https://www.biobots.io/wp-content/uploads/2016/02/BioPCL-specification-sheet.pdf" target="_blank" rel="">PCL specification sheet</a></li> <li style="margin-left: 20px;"><a href="https://www.biobots.io/biowiki/biopcl/" target="_blank">PCL viability data</a></li> </ul> <h3>References</h3> <ol> <li> Kundu, Joydip et al. <a href="https://www.researchgate.net/publication/235371463_An_additive_manufacturing-based_PCL-alginate-chondrocyte_bioprinted_scaffold_for_cartilage_tissue_engineering">An Additive Manufacturing-Based PCL-Alginate-Chondrocyte Bioprinted Scaffold for Cartilage Tissue Engineering</a>. <em>J Tissue Eng Regen Med. </em>2013.</li> </ol>

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