BioBots Reagent Guide
Want some guidance on what BioBots reagents to use with your BioBot 1 bioprinter? Check out the information below to learn more about available reagents to purchase for use with your BioBot 1.
Each of these reagents has a bioreport (our testing results) and protocol for suggested use with the BioBot 1 bioprinter. Many in the community use these reagents to test applications or as controls when developing their own custom reagents! Still have more questions after reading this guide? Contact us at firstname.lastname@example.org.
Materials used for bioprinting present a diverse selection, from hard acellular thermoplastics to cell suspensions completely void of extra material. These materials can be split into three main categories: Matrix, sacrificial and support. The goal of all of these materials is to fabricate viable constructs with high physiological relevance.
The main bioink categories: matrix, sacrificial and support. Learn more about all bioink categories here.) For a comprehensive review of all bioinks for extrusion bioprinting, check out this paper!
|Reagent||Source||Cell Distribution||Resolution (mm)||Sacrificial Support Needed?||Curing Reagent||Documentation||References|
|Alginate||Natural||Good||0.58 ± 0.06||Pluronic F127||Calcium Chloride (CaCl2)||Bioreport Protocol||(6 9–12)|
|Alginate||Natural||Good||0.15 ± 0.03||FRESH||Calcium Chloride (CaCl2)||Bioreport Protocol||(6 9–12)|
|Collagen||Natural||Good||0.32 ± 0.03||FRESH||None||Bioreport Protocol||(13–15)|
|Gelatin Methacrylate (GelMA)||Natural||Good||0.40 ± 0.03||none||BioKey (LAP)||Bioreport Protocol||(16–22)|
|Gelatin/Fibrin Mixture||Natural||Good||0.35 ± 0.03||none||Thrombin/Transglutaminase/Calcium Chloride||Bioreport Protocol|
|Poly (ethylene glycol) diacrylate (PEGDA)||Synthetic||Poor (Try Percoll)||> 0.60||Pluronic F127||BioKey (LAP)||Biopreport Protocol||(1–7)|
Table 1: Matrix reagents, the cell-encapsulating materials in bioprinting, are the key player in the biofabrication process (Learn more about matrix bioinks here). Most matrix bioinks currently developed offer strengths and weaknesses, often meeting some but not all requirements necessary for bioprinting. Reagents in other categories can help combat these weaknesses.
|Reagent||Method for Removal||Matrix Bioink Compatibility||Documentation||References|
|Pluronic F127||Cool (4 °C)||GelMA PEGDA Alginate||Bioreport||(20 25–26 29)|
|FRESH||Heat (37 °C)||Alginate Collagen Type I||Bioreport Protocol||(6 9–12)|
Table 2: Sacrificial bioinks are useful for materials with poor shape fidelity, as well as to create complex negative geometries such as vasculature networks, within a structure. Sacrificial materials, which can be washed away after printing, provide temporary support during the fabrication process.
|Polycaprolactone (PCL)||60 °C||0.16 ± 0.04 mm||Bioreport Protocol||(11 30–31)|
|Poly (lactic-co-glycolic acid) (PLGA)||72 – 77 °C||0.15 ± 0.01 mm||Bioreport Protocol||(32–33)|
Table 3: Support bioinks play a more permanent role in 3D printed constructs and are often used to adjust construct mechanical properties.