Easily Mix Bioinks: The SpeedMixer

Have trouble preparing highly viscous bioinks? Need a tool to mix two components of a bioink with varying viscosities? Or do you need to mix a powder with a viscous solution? Does it take you forever to mix components used in your bioink? If so, we suggest checking out the FlackTek SpeedMixer. We’ve been testing the FlackTek SpeedMixer in our lab here Read More

CAD Designs for Bioprinting: Lattices

Creating 3D designs for bioprinting requires certain considerations to ensure an optimal tool path that will work with complex materials and cell types. Lattices are a common design in bioprinting for testing new materials and parameters in bioprinting, creating a simple 3D porous structure that provides for nutrient transport and waste export. Watch this video Read More

Allevi Publications

Allevi 2 Publications  3D printed UV light cured polydimethylsiloxane devices for drug delivery This paper, published by researchers from  Åbo Akademi University and the University of Helsinki in Finland, use the Allevi 2 platform to fabricate drug-containing PDMS structures. Abstract: The goal of this work was to study the printability of PDMS with a semi-solid extrusion Read More

Custom G-code Tutorial: Dots, Lines, Lattices and Thin Films

Overview While most prints can and should be easily prepared without developing custom gcode through the use of Slic3r, manually editing print files allows users the versatility to adjust and create unique print processes. Slic3r is suggested to prepare print files before attempting custom g-code. Custom g-code is designed for specific use-case scenarios when slic3r is Read More

Common Viability Assays for 3D Cultures

As the 3D culture revolution takes over biology, new methods must be developed to analyze these cultures. Quantitative viability assays for 3D cultures are lacking, and even the ones that are available have only been tested by manufacturers with spheroid cultures, as opposed to larger cell-encapsulated hydrogel cultures. However, there are some current methods for viability analysis that Read More

Creating 3D Controls: Bioprint Study

New to the world of 3D biology, or testing out a new matrix material with your BioBot? This protocol is part of a series to help you learn how to design experiments and easily build with life! Before you begin bioprinting, you need to make sure you have proper controls. Typical 2D culture controls are Read More

Troubleshooting Issues With 3D Culture Viability

Nothing is quite as frustrating as an unexpected loss of viability when working with 3D cultures. Below is a troubleshooting guide that outlines the most common variables affecting viability in 3D cultures and what steps to take to create viable, healthy 3D bioprinted cultures. Part 1: General 3D Culture Variables Before focusing on parameters in the Read More

Soft Tissue Kit Protocol

Overview Figure 1:  Live/dead image of human neonatal dermal fibroblasts encapsulated in bioprinted Gelatin/Fibrin Processed Gelatin/Fibrin Soft Tissue Kit contains all the materials needed to print with this gelatin-based hydrogel, which solidifies through enzymatic crosslinking with a thrombin, calcium chloride, and transglutaminase solution. The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that Read More

Processed Gelatin/Fibrin Bioprinted Viability

Introduction Matrix bioinks, or cell-encapsulating bioinks, are arguably the most important reagents used in the bioprinting processes. These materials must shield cells from shear stresses during the printing process, closely mimic the extracellular matrix and offer quick, nontoxic gelation for optimal print resolution. Types of matrix bioinks range from simple cell slurries suspended in media Read More

Bioprinted Collagen Viability

Introduction LifeInk200 is a high concentration collagen bioink useful for bioprinting structures with high shape fidelity. As the most common protein in many tissues’ ECMs, collagen-based bioinks are great for cell encapsulation because they demonstrate increased cell adhesion (1). As a result of thermal crosslinking, the collagen molecules form fibrils and then more stable and Read More